| 產(chǎn)品編號 | bs-2367R |
| 英文名稱(chēng) | ganglioside GM1 Rabbit pAb |
| 中文名稱(chēng) | 神經(jīng)節苷酯抗體 |
| 別 名 | GM1 ganglioside; Monosialoanglioside; ganglioside GM1. 神經(jīng)節苷脂; |
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Specific References (1) | bs-2367R has been referenced in 1 publications.
[IF=11.467] Melani Solomon. et al. Altered blood-brain barrier transport of nanotherapeutics in lysosomal storage diseases. J CONTROL RELEASE. 2022 Sep;349:1031 FC ; Human.
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| 產(chǎn)品類(lèi)型 | 小分子抗體 |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細胞表面分子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 1.57 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated ganglioside GM1 purified from pig |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function. Subcellular Location: Plasma membrane 含有唾液酸的鞘脂類(lèi)?？藗惪耍‥.Klenk，1935）首先發(fā)現在患Tay－sachs?。ㄌ┧_氏幼年型黑白癡?。┑男耗X中有蓄積（Tag－Sachs gangli-oside），在腦灰白質(zhì)中含量很多，所以對這類(lèi)糖脂命名為神經(jīng)節苷脂。已知糖部分是由己糖、氨基糖、唾液酸組成的腦神經(jīng)節苷脂有8種以上。由山川民夫（1951）在馬血球基質(zhì)中找到的非神經(jīng)系統的神經(jīng)節苷脂——主要是血苷脂[hematoside，結構為神經(jīng)酰胺（1←1β）GLc（4←1β）Gal（3←2α）唾液酸]。神經(jīng)節苷脂較多地存在于細胞膜，以其與膜功能的關(guān)系被引起重視。破傷風(fēng)菌和霍亂菌的外毒素可與特定的神經(jīng)節苷脂分子特異性結合。在水和有機溶劑中可溶。在水中是以數百萬(wàn)個(gè)分子聚合的微團狀態(tài)存在。 神經(jīng)節苷脂對神經(jīng)再生有重大促進(jìn)作用。加拿大金爵集團腦科學(xué)研究室首席科學(xué)家Gatte Walker博士進(jìn)一步研究發(fā)現，神經(jīng)生長(cháng)因子（NGF）和神經(jīng)節苷脂（GM）是腦神經(jīng)再生發(fā)育必需物質(zhì)，GM介導NGF數十倍增強其活性，形成新的豐富的神經(jīng)網(wǎng)絡(luò )，修復并促使腦神經(jīng)再次發(fā)育。 GM能夠介導神經(jīng)生長(cháng)因子NGF促進(jìn)腦神經(jīng)細胞再生，表現出良好的生物效應?！段靼步煌ㄡt科大學(xué)學(xué)報》。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GM1(GS) Polyclonal Antibody, Unconjugated(bs-2367R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:RSC96 (blue). Primary Antibody: Rabbit Anti-ganglioside GM1/AF647 Conjugated antibody (bs-2367R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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