| 產(chǎn)品編號 | bs-9459R |
| 英文名稱(chēng) | HAND1 Rabbit pAb |
| 中文名稱(chēng) | 心臟和神經(jīng)嵴衍生蛋白1抗體 |
| 別 名 | autonomic nervous system and neural crest derivatives-expressed protein 1; heart; Basic helix loop helix transcription factor HAND1; Class A basic helix-loop-helix protein 27; Extraembryonic tissues; Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1; HAND 1; HAND1; HAND-1; HAND1_HUMAN; Heart and neural crest derivatives expressed 1; Heart and neural crest derivatives expressed protein 1; Heart-and neural crest derivatives-expressed protein 1; Hxt; Thing 1; Thing1. |
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Specific References (2) | bs-9459R has been referenced in 2 publications.
[IF=12.153] Tang, Chao. et al. RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy. EXP MOL MED. 2023 Jan;:1-13 WB ; Human.
[IF=4.275] Haibin Zhu. et al. Human HAND1 Inhibits the Conversion of Cholesterol to Steroids in Trophoblasts. J Genet Genomics. 2021 Aug;: IP ; Human.
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| 研究領(lǐng)域 | 心血管 發(fā)育生物學(xué) 干細胞 轉錄調節因子 表觀(guān)遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Dog (predicted: Rat,Rabbit,Pig,Sheep,Cow,Chicken) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HAND1: 101-200/215 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes.dHAND (for deciduum, heart, autonomic nervous system and neural crest derivatives; also designated HAND2) and eHAND (also designated HAND1, HXT or Thing1) are members of a subclass of basic-helix-loop-helix transcription factors that are involved in cardiac development. dHAND and eHAND are expressed in the heart after cardiac looping, and they participate in left-right cardiac asymmetry. dHAND is expressed predominantly on the right side of the looped heart tube and in the pulmonary ventricle, where it activates transcription of various genes, including Ufd1 (for ubiquitin fusion degradation) and Cdc45. In addition, dHAND is expressed in sympathetic neurons and chromafin cells throughout embryonic and fetal development and mediates neural crest development. eHAND expression is primarily observed on the left side and in the systemic ventricle, suggesting that these proteins are involved in the development of segments of the heart tube, which give rise to specific heart chambers during cardiogenesis. Function: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity). Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription factors). Interacts with MDFIC (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Heart. Post-translational modifications: Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity (By similarity). Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: O96004 Gene ID: 9421 Database links: Entrez Gene: 9421 Human Omim: 602406 Human SwissProt: O96004 Human SwissProt: P57100 Rabbit SwissProt: Q28555 Sheep Unigene: 152531 Human Unigene: 224598 Rat Unigene: 39340 Rat |
| 產(chǎn)品圖片 |
Sample:
HL-60(Human) Cell Lysate at 30 ug
Primary: Anti- HAND1 (bs-9459R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 25 kD
Sample:
Placenta (Mouse) Lysate at 40 ug
HL-60 Cell (Human) Lysate at 40 ug
Primary: Anti-HAND1 (bs-9459R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD
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