| 產(chǎn)品編號 | bsm-41144M |
| 英文名稱(chēng) | Procalcitonin(PCT) Mouse mAb |
| 中文名稱(chēng) | 降鈣素原單克隆抗體 |
| 別 名 | Calcitonin; CALCA; Calcitonin carboxyl-terminal peptide; CCP; Katacalcin; PDN-21; CALC1; |
| 研究領(lǐng)域 | 心血管 免疫學(xué) 信號轉導 生長(cháng)因子和激素 |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Monoclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Procalcitonin: 26-141aa |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. Function: The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 μg/L) of clinical assays. The level of procalcitonin rises in a response to a proinflammatory stimulus, especially of bacterial origin. It does not rise significantly with viral or non-infectious inflammations. With the derangements that a severe infection with an associated systemic response brings, the blood levels of procalcitonin may rise to 100 μg/L. In serum, procalcitonin has a half-life of 25 to 30 hours. Remarkably the high procalcitonin levels produced during infections are not followed by a parallel increase in calcitonin or a decrease in serum calcium levels. Subcellular Location: Secreted. DISEASE: Sepsis: Measurement of procalcitonin can be used as a marker of severe sepsis caused by bacteria and generally grades well with the degree of sepsis, although levels of procalcitonin in the blood are very low. PCT has the greatest sensitivity (85%) and specificity (91%) for differentiating patients with systemic inflammatory response syndrome (SIRS) from those with sepsis, when compared with IL-2, IL-6, IL-8, CRP and TNF-alpha. Evidence is emerging that procalcitonin levels can reduce unnecessary antibiotic prescribing to people with lower respiratory tract infections. Currently, procalcitonin assays are widely used in the clinical environment. Pneumonia: Procalcitonin levels may be useful to distinguish bacterial infections from nonbacterial infections. This may help guide antibiotic use, which can help save on cost and drug resistance. Kidney disease: Patients with chronic kidney disease and end-stage renal disease are at higher risk for infections, and procalcitonin has been studied in these populations, who often have higher levels. Procalcitonin can be dialyzed, and so levels are dependent upon when patients receive hemodialysis. While there is no formally accepted cutoff value for patients undergoing HD, using a value of greater or equal to 0.5 ng/mL yielded a sensitivity of 97-98% and a specificity of 70-96%. Hepatitis: PCT, possibly together with CRP, is used to corroborate the MELD score. Similarity: Belongs to the calcitonin family. SWISS: P01258 Gene ID: 796 Database links:
Entrez Gene: 796 Human Omim: 114130 Human SwissProt: P01258 Human Unigene: 37058 Human 降鈣素(CT)是甲狀腺濾泡旁細胞(C細胞)分泌的含有32個(gè)氨基酸的單鏈多肽，可使血鈣、磷水平降低的一種多肽激素，腦組織和垂體含有降鈣素，前列腺等其他組織也存在CT。主要用于C細胞增生和甲狀腺髓樣癌的診斷、也可用于其他類(lèi)型的神經(jīng)內分泌腫瘤的研究。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Procalcitonin(PCT)) Monoclonal Antibody, Unconjugated (bsm-41144M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
4% Paraformaldehyde-fixed TT (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Procalcitonin(PCT)) monoclonal Antibody, unconjugated (bsm-41144M) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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