| 產(chǎn)品編號 | bs-2753R-Bio |
| 英文名稱(chēng) | Rabbit Anti-CENPA/Biotin Conjugated antibody |
| 中文名稱(chēng) | 生物素標記的著(zhù)絲粒蛋白A |
| 別 名 | CENP A; CENP-A; Centromere autoantigen A; Centromere protein A 17kDa; centromere protein A; centromeric protein A; Histone H3 like centromeric protein A; cenpa; CENPA_HUMAN; Histone H3-like centromeric protein A. |
| 規格價(jià)格 | 100ul/2980元 購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul |
| 研究領(lǐng)域 | 細胞生物 染色質(zhì)和核信號 細胞周期蛋白 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 | WB=1:50-200 ELISA=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CENPA C-terminus |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]. Function: Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro). Subunit: Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts (via CATD domain) with HJURP; the interaction is direct and is required for its localization to centromeres. Interacts with CENPC, CENPN and CENPT; interaction is direct. Interacts directly with herpes virus HSV-1 ICP0 protein. Part of a centromere complex consisting of CENPA, CENPT and CENPW. Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height. Tissue Specificity: Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway. Phosphorylation of Ser-7 by AURKA and AURKB during prophase is required for localization of AURKA and AURKB at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by AURKA and is maintained by AURKB. Poly-ADP-ribosylated by PARP1. Similarity: Belongs to the histone H3 family. Database links: Entrez Gene: 1058 Human Omim: 117139 Human SwissProt: P49450 Human Unigene: 1594 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CENP-A可將著(zhù)絲粒變成一個(gè)DNA和蛋白的復合物，而且確保著(zhù)絲粒在細胞分裂中完好無(wú)損。 CENP-A的存在確保了人體有幾乎完全相同的染色體組。 CENP-A功能失常將導致細胞分裂過(guò)程中發(fā)生染色體分離異常。 CENP-A對于染色體著(zhù)絲粒的位置起決定作用，因而被認為是重要的表觀(guān)遺傳學(xué)標記蛋白。 CENP-A改變了核小體的性狀，使得它變得更加堅硬。核小體是由DNA與組合蛋白八聚體構成的真核染色體的一種重復結構，核小體的組織對于基因調控極為重要。不同于染色質(zhì)上的其他區域。 CENP-A取代組蛋白H3形成核小體，CENP-A核小體重復拷貝形成一個(gè)特異的表觀(guān)遺傳區域。 |
