| 產(chǎn)品編號 | bs-0639R-Gold |
| 英文名稱(chēng) | Rabbit Anti-GFP/Gold Conjugated antibody |
| 中文名稱(chēng) | 膠體金標記的綠色熒光蛋白抗體 |
| 別 名 | Green Fluorecent protein. |
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Journal
PMID
IF
Application
[IF=11.9] Radics, Julia, Lisa K?nigsmaier, and Thomas C. Marlovits. "Structure of a pathogenic type 3 secretion system in action." Nature structural & molecular biology (2014). Immunogold labeling of needle complexes with substrates ;
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| 規格價(jià)格 | 100ul/2980元 購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul（10nm 15nm 35nm） |
| 產(chǎn)品類(lèi)型 | 標簽抗體 |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Recombinant protein |
| 產(chǎn)品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from belt jellyfish GFP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Subunit: Monomer. Tissue Specificity: Photocytes. Post-translational modifications: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Similarity: Belongs to the GFP family. Database links:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 該抗體只用于免疫組織化學(xué)、免疫熒光、酶聯(lián)免疫使用。 GFP在科學(xué)研究上有著(zhù)驚人的用途，因為它能夠使我們直接看到細胞內部的運動(dòng)。情況。在任何指定的時(shí)間我們都可以輕易地找出GFP在哪兒：你只需要用紫外光去照射，這時(shí)所有的GFP都將發(fā)出鮮艷的綠色。 不妨做個(gè)實(shí)驗：你把GFP連接到你有興趣觀(guān)察的任何對象上。比如，你可以把它連接到一種病毒上。然后，隨著(zhù)病毒在宿主體內不斷擴散，你就可以通過(guò)跟蹤發(fā)出的綠光來(lái)觀(guān)察病毒的擴散途徑；或者你把它接合到一種蛋白質(zhì)上并通過(guò)顯微鏡觀(guān)察它在細胞內部的移動(dòng)。 GFP是一種現成的熒光蛋白質(zhì)，因此它特別容易使用。大多數可以處理光的蛋白質(zhì)都利用外來(lái)的分子吸收和釋放光子。例如，我們眼睛里的視紫紅質(zhì)利用維生素來(lái)感光。這些“發(fā)光團”必須是專(zhuān)門(mén)為了發(fā)光而生成的，并且被仔細地插入到該蛋白質(zhì)分子內，不同的是，GFP控制光的部位是其自身的一部分，僅由氨基酸構建而成，該部位含有一段三個(gè)氨基酸組成的特殊序列：絲氨酸 Ser－酪氨酸Tyr－甘氨酸 Gly（有時(shí)絲氨酸會(huì )被相似的蘇氨酸取代）。當蛋白質(zhì)鏈折疊時(shí)，這段短片段就被深埋在蛋白質(zhì)內部，然后，發(fā)生一系列化學(xué)反應：甘氨酸與絲氨酸之間形成化學(xué)鍵，生成一個(gè)新的閉合環(huán)，隨后這個(gè)環(huán)會(huì )自動(dòng)脫水。最終，經(jīng)過(guò)大約一個(gè)小時(shí)的反應，周?chē)h(huán)境中的的氧氣攻擊酪氨酸的一個(gè)化學(xué)鍵，形成一個(gè)新的雙鍵并合成熒光發(fā)色團。由于GFP可以形成自己的發(fā)色團，它非常適合于基因工程。你根本不必擔心操作任何奇怪的發(fā)色團，你只需要利用遺傳學(xué)的方法操縱細胞合成GFP蛋白質(zhì)，GFP就會(huì )自動(dòng)折疊并開(kāi)始發(fā)光。 GFP的用途已經(jīng)擴展到藝術(shù)和商務(wù)領(lǐng)域，育種工作者正在探索利用GFP來(lái)創(chuàng )造特殊的熒光植物和各種魚(yú)類(lèi)，GFP已經(jīng)被移植到大鼠、老鼠、青蛙、有翅昆蟲(chóng)、蠕蟲(chóng)以及不計其數的其它生物體內。 |
