| 產(chǎn)品編號 | bs-1186R-FITC |
| 英文名稱(chēng) | Rabbit Anti-SCARB1/Scavenger Receptor BI/FITC Conjugated antibody |
| 中文名稱(chēng) | FITC標記的高密度脂蛋白受體/清道夫受體抗體 |
| 別 名 | HDL-R; High Density Lipoprotein Receptor; CD36 Antigen like 1; CD36L1; CLA 1; CLA1;SR BI; SRB1; SRBI; Scavenger Receptor BI; CD36 AND LIMPII ANALOGOUS 1; CD36 Antigen like 1; CD36L1; CLA 1; CLA1; Collagen type I receptor; MGC138242; SCARB1; Scavebger Receptor Class B Member 1; Scavenger Receptor Class B Type 1; SR BI; SRB1; SRBI; Thrombospondin receptor like 1; High density lipoprotein receptor SR-BI. |
| 規格價(jià)格 | 100ul/2980元 購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul |
| 研究領(lǐng)域 | 免疫學(xué) 生長(cháng)因子和激素 細胞膜受體 糖尿病 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, Chicken, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD36L1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BI (Scavenger Receptor BI) protein binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland. Scavenging Receptor SR-BI plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Function: Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. Subunit: Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. The C-terminal region binds to PDZK1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Membrane, caveola; Multi-pass membrane protein. Note=Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae. Tissue Specificity: Widely expressed. Post-translational modifications: N-glycosylated. The six cysteines of the extracellular domain are all involved in intramolecular disulfide bonds. Similarity: Belongs to the CD36 family. Database links: Entrez Gene: 949 Human Entrez Gene: 20778 Mouse Omim: 601040 Human SwissProt: Q8WTV0 Human SwissProt: Q61009 Mouse Unigene: 520348 Human Unigene: 282242 Mouse Unigene: 88169 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 組織細胞中存在多種高密度脂蛋白受體或高密度脂蛋白結合蛋白，這些受體蛋白的組成、結構及一般特征各不相同；高密度脂蛋白受體在脂質(zhì)代謝中發(fā)揮著(zhù)重要的作用。 |
| 產(chǎn)品圖片 |
Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-SCARB1 antibody (bs-1186R-FITC)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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