| 產(chǎn)品編號 | bs-0429R-AP |
| 英文名稱(chēng) | Rabbit Anti-WNK4/AP Conjugated antibody |
| 中文名稱(chēng) | 堿性磷酸酶（AP）標記的WNK4抗體 |
| 別 名 | PHA 2B; PHA2B; PRKWNK 4; PRKWNK4; Protein kinase lysine deficient 4; Protein kinase with no lysine 4; Serine/threonine protein kinase WNK4; WNK lysine deficient protein kinase 4; WNK4_HUMAN. |
| 規格價(jià)格 | 100ul/2980元 購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul |
| 研究領(lǐng)域 | 心血管 免疫學(xué) 激酶和磷酸酶 通道蛋白 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, ) |
| 產(chǎn)品應用 | WB=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 135kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNK4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+)channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+)secretion to maintain integrated homeostasis. Function: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Subunit: Interacts with the C-terminal region of KCNJ1 (By similarity). Interacts with WNK1 and WNK3 (By similarity). Subcellular Location: Cell junction, tight junction (By similarity). Note=Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity). Tissue Specificity: Expressed in kidney, colon and skin. Post-translational modifications: Phosphorylated by WNK1 and WNK3. DISEASE: Pseudohypoaldosteronism 2B (PHA2B) [MIM:614491]: An autosomal dominant disorder characterized by hypertension, hyperkalemia, hyperchloremia, mild hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 65266 Human Omim: 601844 Human SwissProt: Q96J92 Human Unigene: 105448 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. WNK4蛋白是一種新的絲氨酸/蘇氨酸激酶家族成員的基因，在遠端腎小管表達（腎小管是腎臟的基本結構，在維持人體的水電解質(zhì)平衡方面有著(zhù)重要的作用）而且，這一發(fā)現還有助于人們更好的了解腎臟的生理作用。 WNK4基因的發(fā)現是非常有意義的，WNK4的突變可能會(huì )引起高血壓病的發(fā)生,為人們研究血壓變化的發(fā)生機理提供了一個(gè)線(xiàn)索。 |
