| 產(chǎn)品編號 | bs-13228R-Gold |
| 英文名稱(chēng) | Rabbit Anti-Furin/Gold Conjugated antibody |
| 中文名稱(chēng) | 膠體金標記的弗林蛋白酶抗體 |
| 別 名 | Dibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; FUR; FURIN; FURIN_HUMAN; PACE; Paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzyme; PCSK3; Proprotein convertase subtilisin/kexin type 3; SPC1. |
| 規格價(jià)格 | 100ul/2980元 購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul（10nm 15nm 35nm） |
| 研究領(lǐng)域 | 細胞生物 信號轉導 新陳代謝 泛素 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 74kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Furin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: Furin is a calcium-dependent serine endoprotease that belongs to the subtilisin-like proprotein convertase family. The members of this family process latent precursor proteins into their biologically active products. Furin cleaves at paired basic amino acid processing sites within proparathyroid hormone, transforming growth factor β 1 precursor, proalbumin, pro-β-secretase, membrane type-1 matrix metalloproteinase, β subunit of pro-nerve growth factor and von Willebrand factor. Furin can directly cleave proMMP-2 within the ttrans-Golgi network leading to an inactive form of matrix metalloproteinase-2 (MMP-2). Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The inhibitory mechanism is based on the presence of an inactivating prosegment at the NH2 terminal of the Furin. After initial autocatalytic cleavage, the prosegment remains tightly associated until it reaches the trans-Golgi network where the dissociation of the prosegment and activation of furin occurs. Function: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Subunit: Interacts with FLNA (By similarity). Binds to PACS1 which mediates TGN localization and connection to clathrin adapters. Subcellular Location: Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. Tissue Specificity: Seems to be expressed ubiquitously. Post-translational modifications: The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation. Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. Similarity: Belongs to the peptidase S8 family. Furin subfamily. Contains 1 homo B/P domain. Database links: Entrez Gene: 5045 Human Entrez Gene: 18550 Mouse Omim: 136950 Human SwissProt: P09958 Human SwissProt: P23188 Mouse Unigene: 513153 Human Unigene: 5241 Mouse Unigene: 3220 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 在真核生物細胞中,許多具有生物活性的多肽和蛋白是在其分泌過(guò)程中由前體蛋白經(jīng)內切蛋白酶切割后激活形成的.弗林蛋白酶(Furin)就是這個(gè)內切蛋白酶家族重要成員之一,它可以識別剪切多種蛋白質(zhì),如生長(cháng)醫子、血清蛋白、基質(zhì)金屬蛋白酶、受體、病毒囊膜蛋白和細菌外毒素等.近年來(lái)Furin得到了迅速而廣泛的研究,本文簡(jiǎn)介了它的表達與加工運輸、生物學(xué)功能、與病毒侵染的關(guān)系,以及它的抑制劑. |
